Automated DNA Sizing by Atomic Force Microscopy
نویسنده
چکیده
W a) " NA sizing is one of the most widely used analytical methods in molecular _6 42 biology and biochemistry, and it is a core method for genomic analysis. Many research, • toE health care, and forensic applications rely on DNA sizing. Given the volume of DNA C: sizing done and the associated cost, an increasing need exists for extremely rapid, high. throughput, and inexpensive techniques. Essentially all DNA sizing today is performed Z ~ by gel electrophoresis, although multiple efforts are under way to develop more effective 21P technologies based on entirely new approaches. One such effort is described in this n (D •article: the use of atomic force microscopy in combination with pattern recognition > software to perform DNA sizing. Initial results show that this approach can be c c compared with gel electrophoresis for some applications. Future work aims at CL .developing a practical, high-throughput DNA sizing system. (
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